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KD-Validated Anti-TIMM50 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-TIMM50 Rabbit Monoclonal Antibody AGI1735
    Western blotting analysis using anti-TIMM50 antibody (Cat#62682). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TIMM50 antibody (Cat#62682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-TIMM50 Rabbit Monoclonal Antibody AGI1735
    Western blotting analysis using anti-TIMM50 antibody (Cat#62682). TIMM50 expression in wild type (WT) and TIMM50 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TIMM50 antibody (Cat#62682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-TIMM50 Rabbit Monoclonal Antibody AGI1735
    Flow cytometric analysis of TIMM50 expression in HAP-1 cells using anti-TIMM50 antibody (Cat#62682, 1:2,000). Green, isotype control; red, TIMM50.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q3ZCQ8
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2670
Calculated MW Predicted, 40 kDa , observed , 36 kDa
Gene Name TIMM50
Aliases TIMM50; Translocase Of Inner Mitochondrial Membrane 50; TIM50; TIM50L; Mitochondrial Import Inner Membrane Translocase Subunit TIM50; Translocase Of Inner Mitochondrial Membrane 50 Homolog (Yeast); Homolog Of Yeast Tim50; Tim50-Like Protein; MGCA9
Immunogen A synthesized peptide derived from human TIMM50
Additional Information
Gene ID 92609
Other Names Mitochondrial import inner membrane translocase subunit TIM50, TIMM50, TIM50
Protein Information
Name TIMM50
Synonyms TIM50
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane (PubMed:30190335, PubMed:38828998). Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein
Tissue Location Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level)
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1735
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