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KD-Validated Anti-Sorting Nexin 4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Sorting Nexin 4 Rabbit Monoclonal Antibody AGI1764
    Western blotting analysis using anti-sorting nexin 4 antibody (Cat#AGI1764). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sorting nexin 4 antibody (Cat#AGI1764, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Sorting Nexin 4 Rabbit Monoclonal Antibody AGI1764
    Western blotting analysis using anti-Sorting nexin 4 antibody (Cat#AGI1764). Sorting nexin 4 expression in wild type (WT) and Sorting nexin 4 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Sorting nexin 4 antibody (Cat#AGI1764, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Sorting Nexin 4 Rabbit Monoclonal Antibody AGI1764
    Flow cytometric analysis of Sorting nexin 4 expression in C2C12 cells using anti-Sorting nexin 4 antibody (Cat#AGI1764, 1:2,000). Green, isotype control; red, Sorting nexin 4.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O95219
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2335
Calculated MW Predicted, 52 kDa , observed , 52 kDa
Gene Name SNX4
Aliases SNX4; Sorting Nexin 4; ATG24B; Sorting Nexin-4
Immunogen A synthesized peptide derived from human SNX4
Additional Information
Gene ID 8723
Other Names Sorting nexin-4, SNX4 {ECO:0000303|PubMed:9819414, ECO:0000312|HGNC:HGNC:11175}
Protein Information
Name SNX4 {ECO:0000303|PubMed:9819414, ECO:0000312|HGNC:HGNC:11175}
Function Involved in the regulation of endocytosis and in several stages of intracellular trafficking (PubMed:12668730, PubMed:17994011, PubMed:32513819, PubMed:33468622). Plays a role in recycling endocytosed transferrin receptor and prevent its degradation (PubMed:17994011). Involved in autophagosome assembly by regulating trafficking and recycling of phospholipid scramblase ATG9A (PubMed:32513819, PubMed:33468622).
Cellular Location Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also detected on a juxtanuclear endocytic recycling compartment (ERC).
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1764
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