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KD-Validated Anti-Cyclin E2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cyclin E2 Rabbit Monoclonal Antibody AGI1855
    Western blotting analysis using anti-cyclin E2 antibody (Cat#AGI1855). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin E2 antibody (Cat#AGI1855, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Cyclin E2 Rabbit Monoclonal Antibody AGI1855
    Western blotting analysis using anti-cyclin E2 antibody (Cat#AGI1855). Cyclin E2 expression in wild-type (WT) and cyclin E2 (CCNE2) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin E2 antibody (Cat#AGI1855, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-Cyclin E2 Rabbit Monoclonal Antibody AGI1855
    Immunocytochemical staining of Hela cells with cyclin E2 antibody (Cat#AGI1855, 1:1,000). Nuclei were stained blue with DAPI; Cyclin E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession O96020
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4750
Calculated MW Predicted, 47 kDa, observed, 47 kDa
Gene Name CCNE2
Aliases CCNE2; Cyclin E2; CYCE2; G1/S-Specific Cyclin-E2
Immunogen A synthesized peptide derived from human Cyclin E2
Additional Information
Gene ID 9134
Other Names G1/S-specific cyclin-E2, CCNE2
Protein Information
Name CCNE2
Function Essential for the control of the cell cycle at the late G1 and early S phase.
Cellular Location Nucleus.
Tissue Location According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1855
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