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KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody AGI1856
    Western blotting analysis using anti-CDC123 antibody (Cat#63261). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC123 antibody (Cat#63261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody AGI1856
    Western blotting analysis using anti-CDC123 antibody (Cat#63261). CDC123 expression in wild-type (WT) and CDC123 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDC123 antibody (Cat#63261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody AGI1856
    Flow cytometric analysis of CDC123 expression in HAP-1 cells using anti-CDC123 antibody (Cat#63261, 1:2,000). Green, isotype control; red,CDC123.
    detail
  • ICC - KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody AGI1856
    Immunocytochemical staining of HAP1 cells with anti-CDC123 antibody (Cat#63261, 1:1,000). Nuclei were stained blue with DAPI;CDC123 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O75794
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4760
Calculated MW Predicted, 39 kDa, observed, 39 kDa
Gene Name CDC123
Aliases CDC123; Cell Division Cycle 123; D123; Cell Division Cycle Protein 123 Homolog; C10orf7; HT-1080; PZ32; Cell Division Cycle 123 Homolog (S. Cerevisiae); Chromosome 10 Open Reading Frame 7; Cell Division Cycle 123 Homolog; Protein D123; C10ORF7
Immunogen A synthesized peptide derived from human CDC123
Additional Information
Gene ID 8872
Other Names Translation initiation factor eIF2 assembly protein, Cell division cycle protein 123 homolog, Protein D123, HT-1080, PZ32, CDC123, C10orf7, D123
Protein Information
Name CDC123
Synonyms C10orf7, D123
Function ATP-dependent protein-folding chaperone for the eIF2 complex (PubMed:35031321, PubMed:37507029). Binds to the gamma subunit of the eIF2 complex which allows the subunit to assemble with the alpha and beta subunits (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q62834}.
Tissue Location Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the highest expression in testis.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1856
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