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KD-Validated Anti-Doublecortin Like Kinase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Doublecortin Like Kinase 1 Rabbit Monoclonal Antibody AGI1861
    Western blotting analysis using anti-doublecortin like kinase 1 antibody (Cat#63270). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-doublecortin like kinase 1 antibody (Cat#63270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-Doublecortin Like Kinase 1 Rabbit Monoclonal Antibody AGI1861
    Western blotting analysis using anti-doublecortin like kinase 1 antibody (Cat#63270). Doublecortin like kinase 1 expression in wild-type (WT) and doublecortin like kinase 1 (DCLK1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-doublecortin like kinase 1 antibody (Cat#63270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Doublecortin Like Kinase 1 Rabbit Monoclonal Antibody AGI1861
    Flow cytometric analysis of doublecortin like kinase 1 expression in C2C12 cells using anti-doublecortin like kinase 1 antibody (Cat#63270, 1:2,000). Green, isotype control; red, doublecortin like kinase 1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O15075
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4805
Calculated MW Predicted, 82 kDa, observed, 75 kDa
Gene Name DCLK1
Aliases DCLK1; Doublecortin Like Kinase 1; DCDC3A; KIAA0369; DCAMKL1; DCLK; Doublecortin Domain-Containing Protein 3A; Doublecortin-Like And CAM Kinase-Like 1; Serine/Threonine-Protein Kinase DCLK1; Doublecortin-Like Kinase 1; EC 2.7.11.1; Doublecortin And CaM Kinase-Like 1; EC 2.7.11; CLICK1; CL1
Immunogen A synthesized peptide derived from human DCAMKL1
Additional Information
Gene ID 9201
Other Names Serine/threonine-protein kinase DCLK1, 2.7.11.1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1, DCLK1, DCAMKL1, DCDC3A, KIAA0369
Protein Information
Name DCLK1
Synonyms DCAMKL1, DCDC3A, KIAA0369
Function Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Tissue Location In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1861
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