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KD-Validated Anti-DFFA Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DFFA Rabbit Monoclonal Antibody AGI1862
    Western blotting analysis using anti-DFFA antibody (Cat#63271). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DFFA antibody (Cat#63271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DFFA, DNA fragmentation factor subunit alpha.
    detail
  • WB - KD-Validated Anti-DFFA Rabbit Monoclonal Antibody AGI1862
    Western blotting analysis using anti-DFFA antibody (Cat#63271). DFFA expression in wild-type (WT) and DFFA shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DFFA antibody (Cat#63271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-DFFA Rabbit Monoclonal Antibody AGI1862
    Flow cytometric analysis of DFFA expression in HeLa cells using anti-DFFA antibody (Cat#63271, 1:2,000). Green, isotype control; red, DFFA.
    detail
  • ICC - KD-Validated Anti-DFFA Rabbit Monoclonal Antibody AGI1862
    Immunocytochemical staining of Hela cells with DFFA antibody (Cat#63271, 1:1,000). Nuclei were stained blue with DAPI; DFFA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O00273
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4810
Calculated MW Predicted, 37 kDa, observed, 40 kDa
Gene Name DFFA
Aliases DFFA; DNA Fragmentation Factor Subunit Alpha; DFF-45; DFF45; ICAD; DFF1; DNA Fragmentation Factor, 45kDa, Alpha Polypeptide; DNA Fragmentation Factor 45KDa Subunit; Inhibitor Of CAD; DNA Fragmentation Factor, 45 KD, Alpha Polypeptide; DNA Fragmentation Factor, 45 KD, Alpha Subunit
Immunogen A synthesized peptide derived from human ICAD
Additional Information
Gene ID 1676
Other Names DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45
Protein Information
Name DFFA
Synonyms DFF1, DFF45
Function Inhibitor of the caspase-activated DNase (DFF40).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1862
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