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KD-Validated Anti-ALPP Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ALPP Rabbit Monoclonal Antibody AGI1888
    Western blotting analysis using anti-ALPP antibody (Cat#63386). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALPP antibody (Cat#63386, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-ALPP Rabbit Monoclonal Antibody AGI1888
    Western blotting analysis using anti-ALPP antibody (Cat#63386). ALPP expression in wild type (WT) and ALPP shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALPP antibody (Cat#63386, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-ALPP Rabbit Monoclonal Antibody AGI1888
    Flow cytometric analysis of ALPP expression in HepG2 cells using anti-ALPP antibody (Cat#63386, 1:2,000). Green, isotype control; red, ALPP.
    detail
  • ICC - KD-Validated Anti-ALPP Rabbit Monoclonal Antibody AGI1888
    Immunocytochemical staining of HepG2 cells with anti-ALPP antibody (Cat#63386, 1:1,000). Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P05187
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB5430
Calculated MW Predicted, 58 kDa, observed, 63 kDa
Gene Name ALPP
Aliases ALPP; Alkaline Phosphatase, Placental; PLAP; PALP; Alkaline Phosphatase, Placental Type; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; Alkaline Phosphatase, Placental (Regan Isozyme); Intestinal-Type Alkaline Phosphatase; Intestinal Alkaline Phosphatase; Placental Alkaline Phosphatase; Alkaline Phosphomonoesterase; Glycerophosphatase; Regan Isozyme; ALPI; ALP; IAP
Immunogen A synthesized peptide derived from human Placental alkaline phosphatase (phospho LAP)
Additional Information
Gene ID 250
Other Names Alkaline phosphatase, placental type, 3.1.3.1, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP (HGNC:439)
Protein Information
Name ALPP (HGNC:439)
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor
Tissue Location Detected in placenta (at protein level).
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1888
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