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KD-Validated Anti-Calpastatin Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-Calpastatin Mouse Monoclonal Antibody AGI1940
    Western blotting analysis using anti-calpastatin antibody (Cat#63547). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpastatin antibody (Cat#63547, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Calpastatin Mouse Monoclonal Antibody AGI1940
    Western blotting analysis using anti-calpastatin antibody (Cat#63547). Calpastatin expression in wild type (WT) and calpastatin (CAST) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-calpastatin antibody (Cat#63547, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P20810
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB6455
Calculated MW Predicted, 77 kDa, observed, 120 kDa
Gene Name CAST
Aliases CAST; Calpastatin; Sperm BS-17 Component; Calpain Inhibitor; MIR583HG; MIR583 Host Gene; NONHSAG041072.2; NONHSAG041079.2; NONHSAG041069.2; NONHSAG041075.2; NONHSAG041074.2; HSALNG0043632; HSALNG0148668; HSALNG0043636; HSALNG0043640; HSALNG0037602; HSALNG0043637; MIR583 Host; Lnc-CAST-10; Lnc-CAST-3; BS-17; PLACK
Immunogen Recombinant protein of human CAST
Additional Information
Gene ID 831
Other Names Calpastatin, Calpain inhibitor, Sperm BS-17 component, CAST
Protein Information
Name CAST
Function Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1940
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