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KD-Validated Anti-HARS1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-HARS1 Mouse Monoclonal Antibody AGI1970
    Western blotting analysis using anti-HARS1 antibody (Cat#63655). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HARS1 antibody (Cat#63655, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-HARS1 Mouse Monoclonal Antibody AGI1970
    Western blotting analysis using anti-HARS1 antibody (Cat#63655). HARS1 expression in wild-type (WT) and HARS1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HARS1 antibody (Cat#63655, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-HARS1 Mouse Monoclonal Antibody AGI1970
    Flow cytometric analysis of HARS1 expression in HepG2 cells using anti-HARS1 antibody (Cat#63655, 1:2,000). Green, isotype control; red, HARS1.
    detail
  • ICC - KD-Validated Anti-HARS1 Mouse Monoclonal Antibody AGI1970
    Immunocytochemical staining of HepG2 cells with anti-HARS1 antibody (Cat#63655, 1:1,000). Nuclei were stained blue with DAPI; HARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P12081
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB6755
Calculated MW Predicted, 57 kDa, observed, 50 kDa
Gene Name HARS1
Aliases HARS1; Histidyl-TRNA Synthetase 1; HisRS; HARS; Histidine--TRNA Ligase, Cytoplasmic; Histidyl-TRNA Synthetase; EC 6.1.1.21; USH3B; HRS; Histidine TRNA Ligase 1, Cytoplasmic; Histidine Translase; Usher Syndrome 3B; Jo‐1 Antigen; Jo-1 Antigen; CMT2W
Immunogen Recombinant protein of human HARS
Additional Information
Gene ID 3035
Other Names Histidine--tRNA ligase, cytoplasmic, 6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1 (HGNC:4816), HARS, HRS
Protein Information
Name HARS1 (HGNC:4816)
Synonyms HARS, HRS
Function Catalyzes the ATP-dependent ligation of histidine to the 3'- end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed:29235198). Plays a role in axon guidance (PubMed:26072516).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:F1Q5D5}.
Tissue Location Brain, heart, liver and kidney.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1970
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