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KD-Validated Anti-BCAT1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-BCAT1 Mouse Monoclonal Antibody AGI2010
    Western blotting analysis using anti-BCAT1 antibody (Cat#63802). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCAT1 antibody (Cat#63802, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-BCAT1 Mouse Monoclonal Antibody AGI2010
    Western blotting analysis using anti-BCAT1 antibody (Cat#63802). BCAT1 expression in wild type (WT) and BCAT1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BCAT1 antibody (Cat#63802, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-BCAT1 Mouse Monoclonal Antibody AGI2010
    Flow cytometric analysis of BCAT1 expression in C2C12 cells using anti-BCAT1 antibody (Cat#63802, 1:1,000). Green, isotype control; red, BCAT1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P54687
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB7945
Calculated MW Predicted, 43 kDa, observed, 43 kDa
Gene Name BCAT1
Aliases BCAT1; Branched Chain Amino Acid Transaminase 1; BCATc; BCT1; Branched-Chain-Amino-Acid Aminotransferase, Cytosolic; Branched Chain Aminotransferase 1, Cytosolic; EC 2.6.1.42; ECA39; Branched Chain Amino-Acid Transaminase 1, Cytosolic; Placental Protein 18; Protein ECA39; PNAS121; BCAT(C); MECA39; PP18
Immunogen Recombinant protein of human BCAT1
Additional Information
Gene ID 586
Other Names Branched-chain-amino-acid aminotransferase, cytosolic, BCAT(c), 2.6.1.42, BCAT1, BCT1, ECA39 {ECO:0000303|PubMed:8692959}
Protein Information
Name BCAT1
Synonyms BCT1, ECA39 {ECO:0000303|PubMed:8692959}
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P54690}.
Tissue Location During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2010
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