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KD-Validated Anti-CBX8 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-CBX8 Mouse Monoclonal Antibody AGI2014
    Western blotting analysis using anti-chromobox 8 antibody (Cat#63809). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 8 antibody (Cat#63809, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-CBX8 Mouse Monoclonal Antibody AGI2014
    Western blotting analysis using anti-chromobox 8 antibody (Cat#63809). Chromobox 8 expression in wild-type (WT) and chromobox 8 (CBX8) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 8 antibody (Cat#63809, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-CBX8 Mouse Monoclonal Antibody AGI2014
    Flow cytometric analysis of Chromobox 8 expression in HeLa cells using anti-Chromobox 8 antibody (Cat#63809, 1:1,000). Green, isotype control; red, Chromobox 8.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q9HC52
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 24GB7980
Calculated MW Predicted, 43 kDa, observed, 43 kDa
Gene Name CBX8
Aliases CBX8; Chromobox 8; RC1; PC3; Chromobox Protein Homolog 8; Rectachrome 1; Polycomb 3; HPC3; Chromobox Homolog 8 (Pc Class Homolog, Drosophila); Chromobox Homolog 8 (Drosophila Pc Class); Pc Class 3 Homolog (Drosophila); Chromobox Homolog 8; Pc Class 3 Homolog; Polycomb 3 Homolog; HPc3; Pc3
Immunogen Recombinant protein of human CBX8
Additional Information
Gene ID 57332
Other Names Chromobox protein homolog 8, Polycomb 3 homolog, Pc3, hPc3, Rectachrome 1, CBX8, PC3, RC1
Protein Information
Name CBX8
Synonyms PC3, RC1
Function Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2014
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