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KD-Validated Anti-Epoxide Hydrolase 1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-Epoxide Hydrolase 1 Mouse Monoclonal Antibody AGI2036
    Western blotting analysis using anti-epoxide hydrolase 1 antibody (Cat#63860). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epoxide hydrolase 1 antibody (Cat#63860, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Epoxide Hydrolase 1 Mouse Monoclonal Antibody AGI2036
    Western blotting analysis using anti-epoxide hydrolase 1 antibody (Cat#63860). Epoxide hydrolase 1 expression in wild-type (WT) and epoxide hydrolase 1 (EPHX1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epoxide hydrolase 1 antibody (Cat#63860, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P07099
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB8230
Calculated MW Predicted, 53 kDa, observed, 48 kDa
Gene Name EPHX1
Aliases EPHX1; Epoxide Hydrolase 1; EPHX; Epoxide Hydrolase 1, Microsomal (Xenobiotic); Epoxide Hydratase; EPOX; MEH; Epoxide Hydrolase 1 Microsomal; Microsomal Epoxide Hydrolase; EC 3.3.2.9; HYL1
Immunogen Recombinant protein of human EPHX1
Additional Information
Gene ID 2052
Other Names Epoxide hydrolase 1, 3.3.2.9, EPHX1 (HGNC:3401), EPHX, EPOX
Protein Information
Name EPHX1 (HGNC:3401)
Synonyms EPHX, EPOX
Function Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (By similarity). Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids (PubMed:22798687). Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol (PubMed:24958911). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
Cellular Location Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P07687}; Single-pass type III membrane protein {ECO:0000250|UniProtKB:P07687}
Tissue Location Found in liver..
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2036
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