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KD-Validated Anti-FAF1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-FAF1 Mouse Monoclonal Antibody AGI2039
    Western blotting analysis using anti-fas associated factor 1 antibody (Cat#AGI2039). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fas associated factor 1 antibody (Cat#AGI2039, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-FAF1 Mouse Monoclonal Antibody AGI2039
    Western blotting analysis using anti-fas associated factor 1 antibody (Cat#AGI2039). Fas associated factor 1 expression in wild type (WT) and fas associated factor 1 (FAF1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-fas associated factor 1 antibody (Cat#AGI2039, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-FAF1 Mouse Monoclonal Antibody AGI2039
    Flow cytometric analysis of Fas associated factor 1 expression in HepG2 cells using anti-Fas associated factor 1 antibody (Cat#AGI2039, 1:1,000). Green, isotype control; red, Fas associated factor 1.
    detail
  • ICC - KD-Validated Anti-FAF1 Mouse Monoclonal Antibody AGI2039
    Immunocytochemical staining of HepG2 cells with anti-Fas associated factor 1 antibody (Cat#AGI2039, 1:500). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9UNN5
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB8245
Calculated MW Predicted, 74 kDa, observed, 74 kDa
Gene Name FAF1
Aliases FAF1; Fas Associated Factor 1; UBXD12; UBXN3A; HFAF1; CGI-03; HFAF1s; Fas (TNFRSF6) Associated Factor 1; UBX Domain-Containing Protein 12; UBX Domain-Containing Protein 3A; TNFRSF6-Associated Factor 1; FAS-Associated Factor 1; UBX Domain Protein 3A
Immunogen Recombinant protein of human FAF1
Additional Information
Gene ID 11124
Other Names FAS-associated factor 1, hFAF1, UBX domain-containing protein 12, UBX domain-containing protein 3A, FAF1, UBXD12, UBXN3A
Protein Information
Name FAF1
Synonyms UBXD12, UBXN3A
Function Ubiquitin-binding protein (PubMed:19722279). Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed:26842564). Potentiates but cannot initiate FAS-induced apoptosis (By similarity).
Cellular Location Nucleus.
Tissue Location Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2039
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