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KD-Validated Anti-CWC27 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-CWC27 Mouse Monoclonal Antibody AGI2044
    Western blotting analysis using anti-CWC27 antibody (Cat#63874). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CWC27 antibody (Cat#63874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-CWC27 Mouse Monoclonal Antibody AGI2044
    Western blotting analysis using anti-CWC27 antibody (Cat#63874). CWC27 expression in wild-type (WT) and CWC27 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CWC27 antibody (Cat#63874, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-CWC27 Mouse Monoclonal Antibody AGI2044
    Flow cytometric analysis of Exosome component 1 expression in HepG2 cells using anti-Exosome component 1 antibody (Cat#63872, 1:1,000). Green, isotype control; red, Exosome component 1.
    detail
  • ICC - KD-Validated Anti-CWC27 Mouse Monoclonal Antibody AGI2044
    Immunocytochemical staining of HepG2 cells with anti-CWC27 antibody (Cat#63874, 1:1,000). Nuclei were stained blue with DAPI; CWC27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q6UX04
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB8300
Calculated MW Predicted, 54 kDa, observed, 62 kDa
Gene Name CWC27
Aliases CWC27; CWC27 Spliceosome Associated Cyclophilin; Serologically Defined Colon Cancer Antigen 10; SDCCAG-10; NY-CO-10; SDCCAG10; Probable Inactive Peptidyl-Prolyl Cis-Trans Isomerase CWC27 Homolog; CWC27 Spliceosome Associated Protein Homolog; Spliceosome-Associated Protein CWC27 Homolog; Antigen NY-CO-10; PPIase CWC27; CWC27 Spliceosome-Associated Protein Homolog (S. Cerevisiae); Peptidyl-Prolyl Cis-Trans Isomerase CWC27 Homolog; CWC27 Spliceosome-Associated Protein Homolog; Peptidyl-Prolyl Cis-Trans Isomerase SDCCAG10; PPIase SDCCAG10; RPSKA
Immunogen Recombinant protein of human CWC27
Additional Information
Gene ID 10283
Other Names Spliceosome-associated protein CWC27 homolog, Antigen NY-CO-10, Probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog, PPIase CWC27, Serologically defined colon cancer antigen 10, CWC27 (HGNC:10664)
Protein Information
Name CWC27 (HGNC:10664)
Function As part of the spliceosome, plays a role in pre-mRNA splicing (PubMed:29360106). Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed:20676357). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre- mRNAs (Probable).
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2044
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