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KD-Validated Anti-CDA Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CDA Rabbit Monoclonal Antibody AGI2048
    Western blotting analysis using anti-cytidine deaminase antibody (Cat#63896). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cytidine deaminase antibody (Cat#63896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-CDA Rabbit Monoclonal Antibody AGI2048
    Western blotting analysis using anti-cytidine deaminase antibody (Cat#63896). Cytidine deaminase expression in wild type (WT) and cytidine deaminase (CDA) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cytidine deaminase antibody (Cat#63896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-CDA Rabbit Monoclonal Antibody AGI2048
    Flow cytometric analysis of Cytidine deaminase expression in HepG2 cells using anti-Cytidine deaminase antibody (Cat#63896, 1:2,000). Green, isotype control; red, Cytidine deaminase.
    detail
  • ICC - KD-Validated Anti-CDA Rabbit Monoclonal Antibody AGI2048
    Immunocytochemical staining of HepG2 cells with anti-Cytidine deaminase antibody (Cat#63896, 1:1,000). Nuclei were stained blue with DAPI; Cytidine deaminase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P32320
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB6890
Calculated MW Predicted, 16 kDa, observed, 16 kDa
Gene Name CDA
Aliases CDA; Cytidine Deaminase; CDD; Cytidine Aminohydrolase; Cytosine Nucleoside Deaminase; Small Cytidine Deaminase; EC 3.5.4.5
Immunogen Recombinant protein of human CDA
Additional Information
Gene ID 978
Other Names Cytidine deaminase, 3.5.4.5, Cytidine aminohydrolase, CDA (HGNC:1712), CDD
Protein Information
Name CDA (HGNC:1712)
Synonyms CDD
Function This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
Tissue Location Highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2048
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