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KD-Validated Anti-FUBP3 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-FUBP3 Mouse Monoclonal Antibody AGI2070
    Western blotting analysis using anti-FUBP3 antibody (Cat#64829). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FUBP3 antibody (Cat#64829, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-FUBP3 Mouse Monoclonal Antibody AGI2070
    Western blotting analysis using anti-FUBP3 antibody (Cat #64829). FUBP3 expression in wild-type (WT) and FUBP3 shRNA knockdown (KD) HeLa cells with 10 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FUBP3 antibody (Cat #64829, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
    detail
  • FC - KD-Validated Anti-FUBP3 Mouse Monoclonal Antibody AGI2070
    Flow cytometric analysis of FUBP3 expression in HT-1080 cells using anti-FUBP3 antibody (Cat#64829, 1:2,000). Green, isotype control; red, FUBP3.
    detail
  • ICC - KD-Validated Anti-FUBP3 Mouse Monoclonal Antibody AGI2070
    Immunocytochemical staining of HT-1080 cells with anti-FUBP3 associated protein antibody (Cat#64829, 1:1,000). Nuclei were stained blue with DAPI; FUBP3 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q96I24
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB13150
Calculated MW Predicted, 62 kDa, observed, 62 kDa
Gene Name FUBP3
Aliases FUBP3; Far Upstream Element Binding Protein 3; FBP3; Far Upstream Element (FUSE) Binding Protein 3; Far Upstream Element-Binding Protein 3; FUSE-Binding Protein 3
Immunogen Recombinant protein of human FUBP3
Additional Information
Gene ID 8939
Other Names Far upstream element-binding protein 3, FUSE-binding protein 3, FUBP3, FBP3
Protein Information
Name FUBP3
Synonyms FBP3
Function May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
Cellular Location Nucleus.
Tissue Location Detected in a number of cell lines.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2070
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