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KD-Validated Anti-MSI1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-MSI1 Mouse Monoclonal Antibody AGI2079
    Western blotting analysis using anti-MSI1 antibody (Cat#AGI2079). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MSI1 antibody (Cat#AGI2079, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-MSI1 Mouse Monoclonal Antibody AGI2079
    Western blotting analysis using anti-MSI1 antibody (Cat#AGI2079). MSI1 expression in wild-type (WT) and MSI1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MSI1 antibody (Cat#AGI2079, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O43347
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB13220
Calculated MW Predicted, 39 kDa, observed, 36 kDa
Gene Name MSI1
Aliases MSI1; Musashi RNA Binding Protein 1; RNA-Binding Protein Musashi Homolog 1; Musashi-1; Musashi (Drosophila) Homolog 1; Musashi Homolog 1 (Drosophila); Musashi1
Immunogen Recombinant protein of human MSI1
Additional Information
Gene ID 4440
Other Names RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Protein Information
Name MSI1
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU- 3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q61474}. Nucleus {ECO:0000250|UniProtKB:Q61474}
Tissue Location Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2079
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