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KD-Validated Anti-SPTAN1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-SPTAN1 Mouse Monoclonal Antibody AGI2097
    Western blotting analysis using anti-SPTAN1 antibody (Cat#64875). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-SPTAN1 Mouse Monoclonal Antibody AGI2097
    Western blotting analysis using anti-SPTAN1 antibody (Cat#64875). SPTAN1 expression in wild-type (WT) and SPTAN1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q13813
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB13380
Calculated MW Predicted, 285 kDa, observed, 285 kDa
Gene Name SPTAN1
Aliases SPTAN1; Spectrin Alpha, Non-Erythrocytic 1; Spectrin Alpha Chain, Non-Erythrocytic 1; Spectrin, Non-Erythroid Alpha Subunit; Fodrin Alpha Chain; Alpha-II Spectrin; Alpha-Fodrin; SPTA2; Epididymis Secretory Sperm Binding Protein; Spectrin, Non-Erythroid Alpha Chain; HMND11; DEVEP; EIEE5; HMN11; SPG91; DEE5
Immunogen Recombinant protein of human SPTAN1
Additional Information
Gene ID 6709
Other Names Spectrin alpha chain, non-erythrocytic 1, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit, SPTAN1, NEAS, SPTA2
Protein Information
Name SPTAN1
Synonyms NEAS, SPTA2
Function Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2097
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