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KD-Validated Anti-SELENBP1 Mouse Monoclonal Antibody

Mouse monoclonal Antibody

     
  • WB - KD-Validated Anti-SELENBP1 Mouse Monoclonal Antibody AGI2167
    Western blotting analysis using anti-SELENBP1 antibody (Cat#65041). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SELENBP1 antibody (Cat#65041, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-SELENBP1 Mouse Monoclonal Antibody AGI2167
    Western blotting analysis using anti-SELENBP1 antibody (Cat#65041). SELENBP1 expression in wild-type (WT) and SELENBP1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SELENBP1 antibody (Cat#65041, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q13228
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB13995
Calculated MW Predicted, 52 kDa, observed, 50 kDa
Gene Name SELENBP1
Aliases SELENBP1; Selenium Binding Protein 1; Methanethiol Oxidase; HSBP; LPSB; 56 KDa Selenium-Binding Protein; HSP56; SBP56; SP56; MTO; Epididymis Secretory Sperm Binding Protein Li 134P; Selenium-Binding Protein 1; HEL-S-134P; EC 1.8.3.4; EHMTO; SBP
Immunogen Recombinant protein of human SELENB1
Additional Information
Gene ID 8991
Other Names Methanethiol oxidase, MTO, 1.8.3.4, 56 kDa selenium-binding protein, SBP56, SP56, Selenium-binding protein 1, SELENBP1, SBP
Protein Information
Name SELENBP1
Synonyms SBP
Function Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria (PubMed:29255262). Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport (By similarity).
Cellular Location Nucleus. Cytoplasm, cytosol Membrane {ECO:0000250|UniProtKB:Q8VIF7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8VIF7}. Note=May associate with Golgi membrane (By similarity). May associate with the membrane of autophagosomes (By similarity). {ECO:0000250|UniProtKB:Q8VIF7}
Tissue Location Widely expressed. Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2167
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