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KD-Validated Anti-PHGDH Mouse Monoclonal Antibody

Mouse monoclonal Antibody

     
  • WB - KD-Validated Anti-PHGDH Mouse Monoclonal Antibody AGI2171
    Western blotting analysis using anti-phosphoglycerate dehydrogenase antibody (Cat#AGI2171). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phosphoglycerate dehydrogenase antibody (Cat#AGI2171, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PHGDH Mouse Monoclonal Antibody AGI2171
    Western blotting analysis using anti-phosphoglycerate dehydrogenase antibody (Cat#AGI2171). Phosphoglycerate dehydrogenase expression in wild-type (WT) and phosphoglycerate dehydrogenase (PHGDH) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phosphoglycerate dehydrogenase antibody (Cat#AGI2171, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O43175
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB14070
Calculated MW Predicted, 57 kDa, observed, 57 kDa
Gene Name PHGDH
Aliases PHGDH; PhosphoglyceRate Dehydrogenase; D-3-PhosphoglyceRate Dehydrogenase; SERA; GDH; PDG; 2-OxoglutaRate Reductase; Malate Dehydrogenase; EC 1.1.1.95; 3-PGDH; Epididymis Secretory Protein Li 113; 3-PhosphoglyceRate Dehydrogenase; EC 1.1.1.399; EC 1.1.1.37; HEL-S-113; EC 1.1.1; PHGDHD; 3PGDH; PGDH3; NLS1; PGAD; NLS; PGD
Immunogen Recombinant protein of human PHGDH
Additional Information
Gene ID 26227
Other Names D-3-phosphoglycerate dehydrogenase, 3-PGDH, 1.1.1.95, 2-oxoglutarate reductase, 1.1.1.399, Malate dehydrogenase, 1.1.1.37, PHGDH, PGDH3
Protein Information
Name PHGDH
Synonyms PGDH3
Function Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L- serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2171
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