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KD-Validated Anti-TRIM47 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-TRIM47 Mouse Monoclonal Antibody AGI2187
    Western blotting analysis using anti-TRIM47 antibody (Cat#AGI2187). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM47 antibody (Cat#AGI2187, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-TRIM47 Mouse Monoclonal Antibody AGI2187
    Western blotting analysis using anti-TRIM47 antibody (Cat#AGI2187). TRIM47 expression in wild-type (WT) and TRIM47 knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM47 antibody (Cat#AGI2187, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q96LD4
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB15905
Calculated MW Predicted, 70 kDa, observed, 74 kDa
Gene Name TRIM47
Aliases TRIM47; Tripartite Motif Containing 47; RNF100; GOA; Gene Overexpressed In Astrocytoma Protein; Tripartite Motif-Containing Protein 47; E3 Ubiquitin-Protein Ligase TRIM47; RING Finger Protein 100; Tripartite Motif-Containing 47; EC 2.3.2.27
Immunogen Recombinant protein of human TRIM47
Additional Information
Gene ID 91107
Other Names E3 ubiquitin-protein ligase TRIM47, 2.3.2.27, Gene overexpressed in astrocytoma protein, RING finger protein 100, Tripartite motif-containing protein 47, TRIM47 (HGNC:19020), GOA, RNF100
Protein Information
Name TRIM47 (HGNC:19020)
Synonyms GOA, RNF100
Function E3 ubiquitin-protein ligase that mediates the ubiquitination and proteasomal degradation of CYLD.
Cellular Location Cytoplasm. Nucleus
Tissue Location Low expression in most tissues. Higher expression in kidney tubular cells. Overexpressed in astrocytoma tumor cells
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2187
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