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KD-Validated Anti-Legumain Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Legumain Rabbit Monoclonal Antibody AGI2191
    Western blotting analysis using anti-Legumain antibody (Cat#65172). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Legumain antibody (Cat#65172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Legumain Rabbit Monoclonal Antibody AGI2191
    Western blotting analysis using anti-Legumain antibody (Cat#65172). Legumain expression in wild type (WT) and legumain shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Legumain antibody (Cat#65172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Legumain Rabbit Monoclonal Antibody AGI2191
    Flow cytometric analysis of Legumain expression in HeLa cells using Legumain antibody (Cat#65172, 1:2,000). Green, isotype control; red, Legumain.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q99538
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2095
Calculated MW Predicted, 49 kDa, observed, 36,57 kDa
Gene Name LGMN
Aliases LGMN; Legumain; LGMN1; PRSC1; Protease, Cysteine, 1 (Legumain) ; Asparaginyl Endopeptidase; Protease, Cysteine 1; EC 3.4.22.34; Cysteine Protease 1; AEP
Immunogen A synthesized peptide derived from human Legumain
Additional Information
Gene ID 5641
Other Names Legumain, 3.4.22.34, Asparaginyl endopeptidase, AEP, Protease, cysteine 1, LGMN {ECO:0000303|PubMed:30425301, ECO:0000312|HGNC:HGNC:9472}
Protein Information
Name LGMN {ECO:0000303|PubMed:30425301, ECO:0000312|HGNC:HGNC:9472}
Function Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed:9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity).
Cellular Location Lysosome.
Tissue Location Ubiquitous. Particularly abundant in kidney, heart and placenta.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2191
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