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KD-Validated Anti-MARS1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-MARS1 Rabbit Monoclonal Antibody AGI2205
    Western blotting analysis using anti-MARS1 antibody (Cat#65212). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MARS1 antibody (Cat#65212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-MARS1 Rabbit Monoclonal Antibody AGI2205
    Western blotting analysis using anti-MARS1 antibody (Cat#65212). MARS1 expression in wild-type (WT) and MARS1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MARS1 antibody (Cat#65212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-MARS1 Rabbit Monoclonal Antibody AGI2205
    Flow cytometric analysis of MARS1 expression in HT-1080 cells using anti-MARS1 antibody (Cat#65212, 1:2,000). Green, isotype control; red, MARS1.
    detail
  • ICC - KD-Validated Anti-MARS1 Rabbit Monoclonal Antibody AGI2205
    Immunocytochemical staining of HT-1080 cells with anti-MARS1 antibody (Cat#65212, 1:1,000). Nuclei were stained blue with DAPI; MARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P56192
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB185
Calculated MW Predicted, 101 kDa; observed, 95 kDa
Gene Name MARS1
Aliases MARS1; Methionyl-TRNA Synthetase 1; MetRS; Methionine--TRNA Ligase, Cytoplasmic; SPG70; CMT2U; MARS; Methionyl-TRNA Synthetase; EC 6.1.1.10; Methionine TRNA Ligase 1, Cytoplasmic; Cytosolic Methionyl-TRNA Synthetase; ILFS2; MTRNS; ILLD; TTD9; MRS
Immunogen A synthesized peptide derived from human MARS
Additional Information
Gene ID 4141
Other Names Methionine--tRNA ligase, cytoplasmic, 6.1.1.10, Methionyl-tRNA synthetase, MetRS, MARS1 (HGNC:6898), MARS
Protein Information
Name MARS1 (HGNC:6898)
Synonyms MARS
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971).
Cellular Location Cytoplasm, cytosol. Nucleus, nucleolus. Note=Localizes to the nucleolus in proliferative cells but disappears in quiescent cells
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2205
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