MARS Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | P56192 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 101116 Da |
Antigen Region | 872-899 aa |
Gene ID | 4141 |
---|---|
Other Names | Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS |
Target/Specificity | This MARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 872-899 amino acids from the C-terminal region of human MARS. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MARS1 (HGNC:6898) |
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Synonyms | MARS |
Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971). |
Cellular Location | Cytoplasm, cytosol. Nucleus, nucleolus. Note=Localizes to the nucleolus in proliferative cells but disappears in quiescent cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
Methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
References
Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)
Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)
Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)
Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)
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