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KD-Validated Anti-PAIP1 Rabbit Monoclonal Antibody

Rabbit monoclonal Antibody

     
  • WB - KD-Validated Anti-PAIP1 Rabbit Monoclonal Antibody AGI2207
    Western blotting analysis using anti-PAIP1 antibody (Cat#AGI2207). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAIP1 antibody (Cat#AGI2207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PAIP1 Rabbit Monoclonal Antibody AGI2207
    Western blotting analysis using anti-PAIP1 antibody (Cat#AGI2207). PAIP1 expression in wild-type (WT) and PAIP1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAIP1 antibody (Cat#AGI2207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PAIP1 Rabbit Monoclonal Antibody AGI2207
    Flow cytometric analysis of PAIP1 expression in HepG2 cells using anti-PAIP1 antibody (Cat#AGI2207, 1:2,000). Green, isotype control; red, PAIP1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q9H074
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB195
Calculated MW Predicted, 54 kDa, observed, 45-60 kDa
Gene Name PAIP1
Aliases PAIP1; Poly(A) Binding Protein Interacting Protein 1; PABP-Interacting Protein 1; Polyadenylate-Binding Protein-Interacting Protein 1; PAIP-1; Poly(A)-Binding Protein-Interacting Protein 1; PABC1-Interacting Protein 1
Immunogen Recombinant protein of human MTHFR
Additional Information
Gene ID 10605
Other Names Polyadenylate-binding protein-interacting protein 1, PABP-interacting protein 1, PAIP-1, Poly(A)-binding protein-interacting protein 1, PAIP1 (HGNC:16945)
Protein Information
Name PAIP1 (HGNC:16945)
Function Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA- binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2207
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