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KD-Validated Anti-SP3 Mouse Oligoclonal Antibody

Mouse oligoclonal antibody

     
  • WB - KD-Validated Anti-SP3 Mouse Oligoclonal Antibody AGI2227
    Western blotting analysis using anti-SP3 antibody (Cat#AGI2227). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SP3 antibody (Cat#AGI2227, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-SP3 Mouse Oligoclonal Antibody AGI2227
    Western blotting analysis using anti-SP3 antibody (Cat#AGI2227). SP3 expression in wild-type (WT) and SP3 knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SP3 antibody (Cat#AGI2227, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q02447
Reactivity Rat, Human, Mouse
Clonality Oligoclonal
Isotype Mouse IgG
Calculated MW Predicted, 82 kDa; observed, 70,115kDa
Gene Name SP3
Aliases SP3; Sp3 Transcription Factor; SPR-2; Transcription Factor Sp3; GC-Binding Transcription Factor Sp3; Specificity Protein 3; SPR2
Immunogen Recombinant protein of human SP3
Additional Information
Gene ID 6670
Other Names Transcription factor Sp3, SPR-2, SP3
Protein Information
Name SP3
Function Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
Cellular Location Nucleus. Nucleus, PML body. Note=Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies
Tissue Location Ubiquitously expressed.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2227
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