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KD-Validated Anti-Malic Enzyme 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Malic Enzyme 2 Rabbit Monoclonal Antibody AGI2231
    Western blotting analysis using anti-malic enzyme 2 antibody (Cat#65320). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-malic enzyme 2 antibody (Cat#65320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-Malic Enzyme 2 Rabbit Monoclonal Antibody AGI2231
    Western blotting analysis using anti-malic enzyme 2 antibody (Cat#65320). Malic enzyme 2 expression in wild-type (WT) and malic enzyme 2 (ME2) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-malic enzyme 2 antibody (Cat#65320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P23368
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB930
Calculated MW Predicted, 65 kDa; observed, 58 kDa
Gene Name ME2
Aliases ME2; Malic Enzyme 2; NAD-Dependent Malic Enzyme, Mitochondrial; Malate Dehydrogenase (Oxaloacetate-Decarboxylating); Malic Enzyme 2, NAD(+)-Dependent, Mitochondrial; NAD-ME; Pyruvic-Malic Carboxylase; EC 1.1.1.38; EC 1.1.1; ODS1
Immunogen A synthesized peptide derived from human ME2
Additional Information
Gene ID 4200
Other Names NAD-dependent malic enzyme, mitochondrial, NAD-ME, 1.1.1.38, Malic enzyme 2, ME2
Protein Information
Name ME2
Function NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2231
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