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KD-Validated Anti-Phospho-KIF22 (S427) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Phospho-KIF22 (S427) Rabbit Monoclonal Antibody AGI2240
    Western blotting analysis using anti-phospho-KIF22 (S427) antibody (Cat#65473). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-KIF22 (S427) antibody (Cat#65473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Phospho-KIF22 (S427) Rabbit Monoclonal Antibody AGI2240
    Western blotting analysis using anti-phospho-KIF22 (S427) antibody (Cat#65473). Phospho-KIF22 (S427) expression in wild-type (WT) and KIF22 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-KIF22 (S427) antibody (Cat#65473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • ICC - KD-Validated Anti-Phospho-KIF22 (S427) Rabbit Monoclonal Antibody AGI2240
    Immunocytochemical staining of HepG2 cells with anti-Phospho-KIF22 (S427) antibody (Cat#65473, 1:1,000). Nuclei were stained blue with DAPI; Phospho-KIF22 (S427) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q14807
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB1650
Calculated MW Predicted, 73 kDa; observed, 73 kDa
Gene Name KIF22
Aliases KIF22; Kinesin Family Member 22; OBP-1; OBP-2; KNSL4; Kid; Kinesin-Like Protein KIF22; Kinesin-Like Protein 4; Origin Of Plasmid DNA Replication-Binding Protein; Kinesin-Like DNA-Binding Protein Pseudogene; Kinesin-Like DNA-Binding Protein; OriP Binding Protein; Kinesin-Like 4; A-328A3.2; SEMDJL2; OBP; KID
Immunogen A synthesized peptide derived from human KIF22 around the phosphorylation site of S427
Additional Information
Gene ID 3835
Other Names Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4, KIF22, KID, KNSL4
Protein Information
Name KIF22
Synonyms KID, KNSL4
Function Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed:25743205).
Cellular Location Nucleus. Cytoplasm, cytoskeleton
Tissue Location Expressed in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2240
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