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KD-Validated Anti-SNRPC Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-SNRPC Rabbit Monoclonal Antibody AGI2277
    Western blotting analysis using anti-SNRPC antibody (Cat#65623). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SNRPC antibody (Cat#65623, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-SNRPC Rabbit Monoclonal Antibody AGI2277
    Western blotting analysis using anti-SNRPC antibody (Cat#65623). SNRPC expression in wild-type (WT) and SNRPC shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SNRPC antibody (Cat#65623, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P09234
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB4570
Calculated MW Predicted, 17 kDa; observed, 19 kDa
Gene Name SNRPC
Aliases SNRPC; Small Nuclear Ribonucleoprotein Polypeptide C; U1-C; Yhc1; U1 Small Nuclear Ribonucleoprotein C; U1 SnRNP C; U1C; U1 Small Nuclear RNP Specific C; U1 SnRNP Protein C
Immunogen A synthesized peptide derived from human SNRPC
Additional Information
Gene ID 6631
Other Names U1 small nuclear ribonucleoprotein C {ECO:0000255|HAMAP-Rule:MF_03153}, U1 snRNP C {ECO:0000255|HAMAP-Rule:MF_03153}, U1-C {ECO:0000255|HAMAP-Rule:MF_03153}, U1C {ECO:0000255|HAMAP-Rule:MF_03153}, SNRPC {ECO:0000255|HAMAP-Rule:MF_03153}
Protein Information
Name SNRPC {ECO:0000255|HAMAP-Rule:MF_03153}
Function Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.
Cellular Location Nucleus {ECO:0000255|HAMAP-Rule:MF_03153, ECO:0000269|PubMed:2136774}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2277
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