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KD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody AGI2316
    Western blotting analysis using anti-ATP citrate lyase antibody (Cat#68149). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP citrate lyase antibody (Cat#68149, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody AGI2316
    Western blotting analysis using anti-ATP citrate lyase antibody (Cat#68149). ATP citrate lyase expression in wild type (WT) and ATP citrate lyase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ATP citrate lyase antibody (Cat#68149, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody AGI2316
    Flow cytometric analysis of ATP citrate lyase expression in HepG2 cells using ATP citrate lyase antibody (Cat#68149, 1:2,000). Green, isotype control; red, ATP citrate lyase.
    detail
  • ICC - KD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody AGI2316
    Immunocytochemical staining of HepG2 cells with ATP citrate lyase antibody (Cat#68149, 1:1,000). Nuclei were stained blue with DAPI; ATP citrate lyase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P53396
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1620
Calculated MW Predicted, 121 kDa , observed, 121 kDa
Gene Name ACLY
Aliases ACLY; ATP Citrate Lyase; ACL; ATPCL; CLATP; ATP-Citrate (Pro-S-)-Lyase; Citrate Cleavage Enzyme; ATP-Citrate Synthase; EC 2.3.3.8; ATP Citrate Synthase
Immunogen A synthesized peptide derived from human ATP citrate lyase
Additional Information
Gene ID 47
Other Names ATP-citrate synthase, 2.3.3.8, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY
Protein Information
Name ACLY
Function Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2316
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