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Anti-ACLY Antibody

Rabbit polyclonal antibody to ACLY

     
  • WB - Anti-ACLY Antibody AP60530
    Western blot analysis of ACLY expression in C6 (A) whole cell lysates.
    detail
  • IH - Anti-ACLY Antibody AP60530
    Immunohistochemical analysis of ACLY staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-ACLY Antibody AP60530
    Immunofluorescent analysis of ACLY staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IF/IC, IHC
Primary Accession P53396
Other Accession Q91V92
Reactivity Human, Mouse, Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 120839 Da
Additional Information
Gene ID 47
Other Names ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme
Target/Specificity Recognizes endogenous levels of ACLY protein.
Dilution WB~~E (1/5000 - 1/10000), WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IH~~E (1/5000 - 1/10000), WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~E (1/5000 - 1/10000), WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACLY
Function Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACLY. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60530
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