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KD-Validated Anti-MCM7 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-MCM7 Rabbit Monoclonal Antibody AGI2318
    Western blotting analysis using anti-MCM7 antibody (Cat#68157). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM7 antibody (Cat#68157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-MCM7 Rabbit Monoclonal Antibody AGI2318
    Western blotting analysis using anti-MCM7 antibody (Cat#68157). MCM7 expression in wild type (WT) and MCM7 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-MCM7 antibody (Cat#68157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-MCM7 Rabbit Monoclonal Antibody AGI2318
    Flow cytometric analysis of MCM7 expression in HepG2 cells using MCM7 antibody (Cat#68157, 1:2,000). Green, isotype control; red, MCM7.
    detail
  • ICC - KD-Validated Anti-MCM7 Rabbit Monoclonal Antibody AGI2318
    Immunocytochemical staining of HepG2 cells with MCM7 antibody (Cat#68157, 1:1,000). Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P33993
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB925
Calculated MW Predicted, 81 kDa ; Observed, 81 kDa
Gene Name MCM7
Aliases Minichromosome Maintenance Complex Component 7; CDC47; PPP1R104; MCM2; Protein Phosphatase 1, Regulatory Subunit 104; DNA Replication Licensing Factor MCM7; CDC47 Homolog; P1.1-MCM3; MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 7; Minichromosome Maintenance Deficient 7; Homolog Of S. Cerevisiae Cdc47; EC 3.6.4.12; P1CDC47; PNAS146; P85MCM
Immunogen A synthesized peptide derived from human MCM7
Additional Information
Gene ID 4176
Other Names DNA replication licensing factor MCM7, 3.6.4.12, CDC47 homolog, P1.1-MCM3, MCM7 (HGNC:6950), CDC47, MCM2
Protein Information
Name MCM7 (HGNC:6950)
Synonyms CDC47, MCM2
Function Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for S-phase checkpoint activation upon UV-induced damage.
Cellular Location Nucleus. Chromosome. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
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$ 149.00
$ 499.00
Cat# AGI2318
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