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KD-Validated Anti-Annexin V Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Annexin V Rabbit Monoclonal Antibody AGI2372
    Western blotting analysis using anti-Annexin V antibody (Cat#69585). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Annexin V antibody (Cat#69585, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Annexin V Rabbit Monoclonal Antibody AGI2372
    Western blotting analysis using anti-Annexin V antibody (Cat#69585). Annexin V expression in wild type (WT) and Annexin V shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Annexin V antibody (Cat#69585,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Annexin V Rabbit Monoclonal Antibody AGI2372
    Flow cytometric analysis of Annexin V expression in HT-1080 cells using Annexin V antibody (Cat#69585,1:2,000). Green, isotype control; red, Annexin V.
    detail
  • ICC - KD-Validated Anti-Annexin V Rabbit Monoclonal Antibody AGI2372
    Immunocytochemical staining of HT-1080 cells with Annexin V antibody (Cat#69585, 1:1,000). Nuclei were stained blue with DAPI; Annexin V was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P08758
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB650
Calculated MW Predicted, 36 kDa; Observed, 30 kDa
Gene Name ANXA5
Aliases ANXA5; Annexin A5; CPB-I; PAP-I; Placental Anticoagulant Protein I; Vascular Anticoagulant-Alpha; Calphobindin I; Endonexin II; Annexin V; VAC-Alph; RPRGL3; ANX5; ENX2; Placental Anticoagulant Protein 4; Thromboplastin Inhibitor; Anchorin CII; Lipocortin V; VAC-Alpha; Annexin-5; PP4; Epididymis Secretory Protein Li 7; HEL-S-7; CBP-I
Immunogen A synthesized peptide derived from human Annexin V
Additional Information
Gene ID 308
Other Names Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CPB-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PP4, Placental anticoagulant protein I, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, ANXA5, ANX5, ENX2, PP4
Protein Information
Name ANXA5
Synonyms ANX5, ENX2, PP4
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2372
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