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KD-Validated Anti-RRM1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-RRM1 Rabbit Monoclonal Antibody AGI2379
    Western blotting analysis using anti-RRM1 antibody (Cat#AGI2379). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RRM1 antibody (Cat#AGI2379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-RRM1 Rabbit Monoclonal Antibody AGI2379
    Western blotting analysis using anti-RRM1 antibody (Cat#AGI2379). RRM1 expression in wild type (WT) and RRM1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-RRM1 antibody (Cat#AGI2379, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-RRM1 Rabbit Monoclonal Antibody AGI2379
    Flow cytometric analysis of RRM1 expression in HepG2 cells using RRM1 antibody (Cat#AGI2379, 1:2,000). Green, isotype control; red, RRM1.
    detail
  • ICC - KD-Validated Anti-RRM1 Rabbit Monoclonal Antibody AGI2379
    Immunocytochemical staining of HepG2 cells with RRM1 antibody (Cat#AGI2379, 1:1,000). Nuclei were stained blue with DAPI; RRM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P23921
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB960
Calculated MW Predicted, 90 kDa ; Observed, 75 kDa
Gene Name RRM1
Aliases Ribonucleotide Reductase Catalytic Subunit M1; Ribonucleoside-Diphosphate Reductase Large Subunit; Ribonucleoside-Diphosphate Reductase Subunit M1; Ribonucleotide Reductase M1 Polypeptide; EC 1.17.4.1; RR1; Ribonucleotide Reductase Large Subunit; Ribonucleotide Reductase, R1 Subunit; RIR1; R1
Immunogen A synthesized peptide derived from human beta RRM1
Additional Information
Gene ID 6240
Other Names Ribonucleoside-diphosphate reductase large subunit, 1.17.4.1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit, RRM1, RR1
Protein Information
Name RRM1
Synonyms RR1
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2379
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