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KO-Validated Anti-GAPDH Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KO-Validated Anti-GAPDH Mouse Monoclonal Antibody AGI2424
    Western blotting analysis using anti-GAPDH antibody (Cat#71172). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GAPDH antibody (Cat#71172, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KO-Validated Anti-GAPDH Mouse Monoclonal Antibody AGI2424
    Western blotting analysis using anti-GAPDH antibody (Cat#71172). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GAPDH antibody (Cat#71172, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KO-Validated Anti-GAPDH Mouse Monoclonal Antibody AGI2424
    Flow cytometric analysis of GAPDH expression in HepG2 cells using anti-GAPDH antibody (Cat#71172, 1:2,000). Green, isotype control; red, GAPDH.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P04406
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG
Clone Names 24GB7870
Calculated MW Predicted, 36 kDa; observed, 36 kDa
Gene Name GAPDH
Aliases GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; GAPD; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein; OCAS, P38 Component; HEL-S-162eP; EC 2.6.99.-; EC 1.2.1; G3PD
Immunogen A synthesized peptide derived from human GAPDH
Additional Information
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, 1.2.1.12, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141}
Protein Information
Name GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141}
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D- glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Cellular Location Cytoplasm, cytosol. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261}
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$ 149.00
$ 499.00
Cat# AGI2424
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