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Cytokeratin 8/18 Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone C-51 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, FC |
|---|---|
| Primary Accession | P05787 |
| Other Accession | 3856 (CK8), 3875 (CK18), 533782, 708445 (CK8), 406013 (CK18), P05783 (CK18) |
| Reactivity | Human, Monkey, Pig, Bovine, Sheep |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | C-51 |
| Calculated MW | 52.5kDa (CK8); 45kDa (CK18) |
| Gene ID | 3856 |
|---|---|
| Other Names | Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8 |
| Application Note | WB~~1:1000 IHC~~1:100~500 IF~~1:50~200 FC~~1:10~50 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Cytokeratin 8/18 Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KRT8 |
|---|---|
| Synonyms | CYK8 |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Cellular Location | Cytoplasm. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q10758}. Nucleus matrix {ECO:0000250|UniProtKB:Q10758} |
| Tissue Location | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Ā
References
Bartek et al. J. Pathology,164:215-24 (1991). | Bartkova et al. Neoplasma 38(4), 439-46 (1991). |
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