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Anti-ASRGL1 (Asparaginase Like 1) Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | Q7L266 |
| Other Accession | 535326 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1 |
| Clone Names | CRASH/1289 |
| Calculated MW | 32055 Da |
| Gene ID | 80150 |
|---|---|
| Other Names | ALP1; ASGL1; Asparaginase like 1; ASRGL1; beta-aspartyl-peptidase; CRASH; isoaspartyl dipeptidase; isoaspartyl peptidase/L-asparaginase; L asparaginase; L asparagine amidohydrolase |
| Application Note | Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-ASRGL1 (Asparaginase Like 1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ASRGL1 |
|---|---|
| Synonyms | ALP, CRASH |
| Function | Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp- L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine. |
| Cellular Location | Cytoplasm. Note=Midpiece of sperm tail |
| Tissue Location | Expressed in brain, kidney, testis and tissues of the gastrointestinal tract. Present in sperm (at protein level). Over- expressed in uterine, mammary, prostatic and ovarian carcinoma |
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Provided below are standard protocols that you may find useful for product applications.
Background
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.
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