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> home > Products > Primary Antibodies > Antibody Collections > Zebrafish > Polg antibody - N-terminal region
Polg antibody - N-terminal region
Rabbit Polyclonal Antibody
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Application 
| WB |
|---|---|
| Primary Accession | Q9QYV8 |
| Other Accession | NM_053528, NP_445980 |
| Reactivity | Human, Mouse, Rat, Zebrafish, Horse, Yeast, Bovine, Guinea Pig, Dog |
| Predicted | Human, Mouse, Rat, Zebrafish, Pig, Horse, Bovine, Guinea Pig, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 136kDa |
| Gene ID | 85472 |
|---|---|
| Other Names | DNA polymerase subunit gamma-1, 2.7.7.7, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, Polg, Mip1, Polg1 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-Polg antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | Polg antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Polg {ECO:0000312|RGD:620057} |
|---|---|
| Synonyms | Mip1, Polg1 |
| Function | Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates (By similarity). Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double- stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post- insertion site. After completing replication of a mtDNA strand, mediates 3' -> 5' exonucleolytic degradation at the nick to enable proper ligation (By similarity). Highly accurate due to high nucleotide selectivity and 3' -> 5' exonucleolytic proofreading. Proficiently corrects base substitutions, single-base additions and deletions in non-repetitive sequences and short repeats, but displays lower proofreading activity when replicating longer homopolymeric stretches. Exerts exonuclease activity toward single-stranded DNA and double- stranded DNA containing 3'-terminal mispairs. When a misincorporation occurs, transitions from replication to a pro-nucleolytic editing mode and removes the missincorporated nucleoside in the exonuclease active site. Proceeds via an SN2 nucleolytic mechanism in which Asp-198 catalyzes phosphodiester bond hydrolysis and Glu-200 stabilizes the leaving group. As a result the primer strand becomes one nucleotide shorter and is positioned in the post-insertion site, ready to resume DNA synthesis (By similarity). Exerts 5'-deoxyribose phosphate (dRP) lyase activity and mediates repair-associated mtDNA synthesis (gap filling) in base-excision repair pathway. Catalyzes the release of the 5'-terminal 2-deoxyribose-5-phosphate sugar moiety from incised apurinic/apyrimidinic (AP) sites to produce a substrate for DNA ligase. The dRP lyase reaction does not require divalent metal ions and likely proceeds via a Schiff base intermediate in a beta-elimination reaction mechanism (By similarity). |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:P54098}. Mitochondrion matrix, mitochondrion nucleoid {ECO:0000250|UniProtKB:P54098} |
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$ 389.00
Cat# AI12919
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