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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SPEF1 Antibody - N-terminal region   

SPEF1 Antibody - N-terminal region

Rabbit Polyclonal Antibody

  • WB - SPEF1 Antibody - N-terminal region AI16160

    Host: Rabbit
    Target Name: SPEF1
    Sample Tissue: Ovary Tumor lysates
    Antibody Dilution: 1.0μg/ml
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4P9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 25kDa
Additional Information
Gene ID 25876
Alias Symbol SPEF1, C20orf28,
Other Names Sperm flagellar protein 1, SPEF1, C20orf28
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 &mu, l of distilled water. Final Anti-SPEF1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsSPEF1 Antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPEF1
Synonyms C20orf28
Function Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (By similarity). Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia (By similarity). Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs (PubMed:31473225). Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia (By similarity).
Cellular Location Cytoplasm. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q99JL1} Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q0IH24} Apical cell membrane. Basolateral cell membrane. Cytoplasm, cytoskeleton, stress fiber. Cell projection, microvillus. Cell projection, lamellipodium. Cell projection, filopodium. Note=Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia (By similarity). Colocalizes with TJP1 and CGN at sites of cell-cell contact in intestinal epithelial cells (PubMed:31473225) {ECO:0000250|UniProtKB:Q0IH24, ECO:0000269|PubMed:31473225}
Tissue Location Expressed in the intestinal epithelial cells (at protein level).
Research Areas
Citations (0)

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Wiemann S.,et al.Genome Res. 11:422-435(2001).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Chan S.W.,et al.Gene 353:189-199(2005).

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$ 389.00
Cat# AI16160
Availability: 2-3 days
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