SPEF1 Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, IHC-F, IF, ICC |
---|---|
Primary Accession | Q9Y4P9 |
Reactivity | Rat, Pig, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 26987 Da |
Gene ID | 25876 |
---|---|
Other Names | Sperm flagellar protein 1, SPEF1, C20orf28 |
Dilution | Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
Name | SPEF1 |
---|---|
Synonyms | C20orf28 |
Function | Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (By similarity). Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia (By similarity). Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs (PubMed:31473225). Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia (By similarity). |
Cellular Location | Cytoplasm. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q99JL1} Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q0IH24} Apical cell membrane. Basolateral cell membrane. Cytoplasm, cytoskeleton, stress fiber. Cell projection, microvillus. Cell projection, lamellipodium. Cell projection, filopodium. Note=Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia (By similarity). Colocalizes with TJP1 and CGN at sites of cell-cell contact in intestinal epithelial cells (PubMed:31473225) {ECO:0000250|UniProtKB:Q0IH24, ECO:0000269|PubMed:31473225} |
Tissue Location | Expressed in the intestinal epithelial cells (at protein level). |
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