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> home > Products > Primary Antibodies > Antibody Collections > GPCR Antibodies > GPR65 / TDAG8 Antibody (Extracellular Domain)
GPR65 / TDAG8 Antibody (Extracellular Domain)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P |
|---|---|
| Primary Accession | Q8IYL9 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 39kDa |
| Dilution | IHC-P (10 µg/ml) |
| Gene ID | 8477 |
|---|---|
| Other Names | Psychosine receptor, G-protein coupled receptor 65, T-cell death-associated gene 8 protein, GPR65, TDAG8 |
| Target/Specificity | Human GPR65. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except VCL (50%), CPS1 (39%). |
| Reconstitution & Storage | Long term: -70°C; Short term: +4°C |
| Precautions | GPR65 / TDAG8 Antibody (Extracellular Domain) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPR65 {ECO:0000303|PubMed:27287411, ECO:0000312|HGNC:HGNC:4517} |
|---|---|
| Function | Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions (PubMed:15326175, PubMed:15618224, PubMed:20855608, PubMed:33478938, PubMed:37722051, PubMed:39753132). Activated by an optimal pH of 7.4 (PubMed:39753132). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide- binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:15326175, PubMed:15618224, PubMed:37722051, PubMed:39753132). GPR65 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed:15618224, PubMed:37722051, PubMed:39753132). May also act as a receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421, PubMed:15326175). Plays a role in immune response by maintaining lysosome function and regulating T-cell metabolism (PubMed:27287411). Acts as a regulator of inflammation by mediating pH-sensing of extracellular acidification which takes place in inflamed tissues: activation regulates endo-lysosomal function of immune cells and T-cell metabolism (By similarity). Constitutively active in endosomes and stimulates adenylate cyclase production from endosomes independently from extracellular pH changes (PubMed:39753132). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Internalizes and localizes to early and late endosomes, from where GPR65 signals at steady state, irrespective of extracellular pH (PubMed:39753132). Changes in extracellular pH may relocalize receptor signaling to the cell membrane (PubMed:39753132). |
| Tissue Location | Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes |
| Volume | 50 µl |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation- induced cell death or differentiation of T-cells.
References
Kyaw H.,et al.DNA Cell Biol. 17:493-500(1998).
Heilig R.,et al.Nature 421:601-607(2003).
Im D.-S.,et al.J. Cell Biol. 153:429-434(2001).
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$ 467.50
Cat# ALS10422
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