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GAK Antibody (aa101-150)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O14976 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 143kDa |
| Dilution | ELISA (1:5000), IHC-P (20 µg/ml), WB (1:500-1:1000) |
| Gene ID | 2580 |
|---|---|
| Other Names | Cyclin-G-associated kinase, 2.7.11.1, GAK |
| Target/Specificity | GAK Antibody detects endogenous levels of total GAK protein. |
| Reconstitution & Storage | Store at -20°C for up to one year. |
| Precautions | GAK Antibody (aa101-150) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GAK (HGNC:4113) |
|---|---|
| Function | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (PubMed:10625686). May play a role in clathrin-mediated endocytosis and intracellular trafficking, and in the dynamics of clathrin assembly/disassembly (PubMed:18489706). |
| Cellular Location | Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions. Recruitment to clathrin- coated vesicles depends on temporal variations in phosphoinositide composition of clathrin-coated vesicles (PubMed:31962345) |
| Tissue Location | Ubiquitous. Highest in testis. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
References
Kimura S.H.,et al.Genomics 44:179-187(1997).
Greener T.,et al.J. Biol. Chem. 275:1365-1370(2000).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
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