Anti-GAK Antibody
Rabbit polyclonal antibody to GAK
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF/IC, IHC |
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Primary Accession | O14976 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 143191 Da |
Gene ID | 2580 |
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Other Names | Cyclin-G-associated kinase |
Target/Specificity | Recognizes endogenous levels of GAK protein. |
Dilution | WB~~WB (1/200 - 1/500), IH (1/50 - 1/100), IF/IC (1/50 - 1/100) IH~~WB (1/200 - 1/500), IH (1/50 - 1/100), IF/IC (1/50 - 1/100) IF~~WB (1/200 - 1/500), IH (1/50 - 1/100), IF/IC (1/50 - 1/100) |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | GAK (HGNC:4113) |
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Function | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (PubMed:10625686). May play a role in clathrin-mediated endocytosis and intracellular trafficking, and in the dynamics of clathrin assembly/disassembly (PubMed:18489706). |
Cellular Location | Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions. Recruitment to clathrin- coated vesicles depends on temporal variations in phosphoinositide composition of clathrin-coated vesicles (PubMed:31962345) |
Tissue Location | Ubiquitous. Highest in testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GAK. The exact sequence is proprietary.
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