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HLA-E Antibody (clone MEM-E/02)

Mouse Monoclonal Antibody

     
  • IHC-P - HLA-E Antibody (clone MEM-E/02) ALS17223
    Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • IHC-P - HLA-E Antibody (clone MEM-E/02) ALS17223
    Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P13747
Other Accession 3133
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MEM-E/02
Calculated MW 40058 Da
Dilution IHC-P (10 µg/ml),
Additional Information
Gene ID 3133
Other Names HLA-E, EA2.1, HLAE, MHC HLA-E alpha-2.1, MHC HLA-E alpha-1, EA1.2, HLA-6.2, MHC class I antigen E, QA1
Reconstitution & Storage PBS, pH 7.4, 15 mM sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsHLA-E Antibody (clone MEM-E/02) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-E {ECO:0000303|PubMed:9486650, ECO:0000312|HGNC:HGNC:4962}
Function Non-classical major histocompatibility class Ib molecule involved in immune self-nonself discrimination. In complex with B2M/beta-2-microglobulin binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules (VL9 peptides - VMAPRT[V/L][L/V/I/F]L) (PubMed:9754572, PubMed:18083576, PubMed:37264229, PubMed:18339401, PubMed:35705051). Peptide-bound HLA- E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self (PubMed:9754572, PubMed:9486650, PubMed:17179229, PubMed:18083576, PubMed:37264229). Upon cellular stress, preferentially binds signal sequence-derived peptides from stress-induced chaperones and is no longer recognized by NK cell inhibitory receptor KLRD1-KLRC1, resulting in impaired protection from NK cells (PubMed:12461076). Binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules and acts as a ligand for NK cell activating receptor KLRD1-KLRC2, likely playing a role in the generation and effector functions of adaptive NK cells and in maternal- fetal tolerance during pregnancy (PubMed:9754572, PubMed:30134159, PubMed:37264229). Besides self-peptides, can also bind and present pathogen-derived peptides conformationally similar to VL9 peptides to alpha-beta T cell receptor (TCR) on unconventional CD8-positive cytotoxic T cells, ultimately triggering antimicrobial immune response (PubMed:16474394, PubMed:30087334, PubMed:20195504, PubMed:34228645). Presents HIV gag peptides (immunodominant KAFSPEVIPMF and subdominant KALGPAATL epitopes) predominantly to CD8-positive T cell clones expressing a TRAV17-containing TCR, triggering HLA-E-restricted T cell responses (PubMed:34228645). Presents mycobacterial peptides to HLA-E- restricted CD8-positive T cells eliciting both cytotoxic and immunoregulatory functions (PubMed:20195504, PubMed:35705051).
Cellular Location Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane
Tissue Location Expressed in secretory endometrial cells during pregnancy (at protein level). The expression in nonlymphoid tissues is restricted to endothelial cells from all types of vessels, including arteries, veins, capillaries, and lymphatics (at protein level). In lymphoid organs, it is mainly expressed in endothelial venules, B and T cells, monocytes, macrophages, NK cells and megakaryocytes (at protein level).
Research Areas
Citations (0)
citation

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Background

Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules.

References

Mizuno S.,et al.J. Immunol. 140:4024-4030(1988).
Ulbrecht M.,et al.Eur. J. Immunol. 29:537-547(1999).
Ishitani A.,et al.Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
Shiina S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Koller B.H.,et al.J. Immunol. 141:897-904(1988).

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Discontinued
Cat# ALS17223
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