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PLOD1 Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - PLOD1 Antibody (C-term) AM8710b
    All lanes : Anti-PLOD1 Antibody (C-term) at 1:4000 dilution Lane 1: U-87MG whole cell lysate Lane 2: K562 whole cell lysate Lane 3: HeLa whole cell lysate Lane 4: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q02809
Reactivity Human
Predicted Human
Host Mouse
Clonality monoclonal
Isotype IgG2b,κ
Clone Names 2114CT109.4.71.73
Calculated MW 83550 Da
Additional Information
Gene ID 5351
Other Names Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, 1.14.11.4, Lysyl hydroxylase 1, LH1, PLOD1, LLH, PLOD
Target/Specificity This PLOD1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 61-94 amino acids from the C-terminal region of human PLOD1.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLOD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLOD1
Synonyms LLH, PLOD
Function Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys- Gly- sequences in collagens (PubMed:8621606, PubMed:10686424, PubMed:15854030). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
Cellular Location Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side
Research Areas
Citations (0)

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Background

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

References

Hautala T.,et al.Genomics 13:62-69(1992).
Heikkinen J.,et al.Genomics 24:464-471(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Pirskanen A.,et al.J. Biol. Chem. 271:9398-9402(1996).

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$ 325.00
$ 109.00
Cat# AM8710b
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