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Mouse Monoclonal Antibody to DNMT3A
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, E |
|---|---|
| Primary Accession | Q9Y6K1 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4G5F11 |
| Isotype | Mouse IgG2a |
| Calculated MW | 102kDa |
| Description | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.; |
| Immunogen | Purified recombinant fragment of human DNMT3A (AA: 46-180) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 FCM: 1/200 - 1/400 |
| Gene ID | 1788 |
|---|---|
| Other Names | TBRS; DNMT3A2; M.HsaIIIA |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to DNMT3A is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DNMT3A |
|---|---|
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443). DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity). |
| Cellular Location | Nucleus. Chromosome Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin {ECO:0000250|UniProtKB:O88508} |
| Tissue Location | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.PLoS One. 2014 Jun 17;9(6):e93353. ; 2.Asian Pac J Cancer Prev. 2013;14(10):5713-8.;
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$ 385.00
Cat# AO2351a
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