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F2R

Purified Mouse Monoclonal Antibody

     
  • E - F2R AO2642a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - F2R AO2642a
    Figure 2:Western blot analysis using F2R mAb against human F2R (AA: 42-176) recombinant protein. (Expected MW is 41.3 kDa)
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  • WB - F2R AO2642a
    Figure 3:Western blot analysis using F2R mAb against HEK293 (1) and F2R(AA: 42-176)-hIgGFc transfected HEK293 (2) cell lysate.
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  • IHC - F2R AO2642a
    Figure 4:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using F2R mouse mAb with DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P25116
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5E2E6
Isotype Mouse IgG1
Calculated MW 47.4kDa
Immunogen Purified recombinant fragment of human F2R (AA: 42-176) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2149
Other Names TR; HTR; CF2R; PAR1; PAR-1
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
IHC~~ 1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsF2R is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name F2R
Synonyms CF2R, PAR1, TR
Function High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Platelets and vascular endothelial cells.
Research Areas
Citations (0)
citation

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References

1.Respirology. 2015 Nov;20(8):1285-7. 2.J Neurosci. 2015 Jan 14;35(2):776-85.

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$ 385.00
Cat# AO2642a
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