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APAF1
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, E |
|---|---|
| Primary Accession | O14727 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2H9A1 |
| Isotype | Mouse IgG2b |
| Calculated MW | 141.8kDa |
| Immunogen | Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 317 |
|---|---|
| Other Names | CED4; APAF-1 |
| Dilution | E~~ 1/10000 WB~~ 1/500 - 1/2000 FCM~~1/200 - 1/400 ICC~~ 1/100 - 1/500 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | APAF1 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | APAF1 (HGNC:576) |
|---|---|
| Synonyms | KIAA0413 |
| Function | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
1.Tumour Biol. 2014 Mar;35(3):2211-8.2.Cancer Sci. 2011 Jan;102(1):267-74.
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