POTEB Antibody (C-term)

All lanes : Anti-POTEB Antibody (C-term) at 1:2000 dilution Lane 1: Human ovary tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
POTEB antibody (C-term) (Cat. #AP10032b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the POTEB antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
POTEB Antibody (C-term) (Cat. #AP10032b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SPECIFICATION
CITATIONS
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	FC, IHC-P, WB, E
Primary Accession	Q6S5H4
Other Accession	P0CG39, P0CG38, A5A3E0, Q86YR6, B2RU33, H3BUK9, NP_997238.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	381-410 aa

Additional Information
Other Names	POTE ankyrin domain family member B, ANKRD26-like family B member 1, Prostate, ovary, testis-expressed protein on chromosome 15, POTE-15, POTEB, A26B1, POTE15
Target/Specificity	This POTEB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-410 amino acids from the C-terminal region of human POTEB.
Dilution	FC~~1:10~50 IHC-P~~1:50~100 WB~~1:2000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	POTEB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

Submit Review

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Ordering Information

Other Products

Shipping Information