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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Anti-Wnt-Pathway (plus Lgr5) Antibodies   >   FSTL1 Antibody (C-term)   

FSTL1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FSTL1 Antibody (C-term) AP10534b
    All lanes : Anti-FSTL1 Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - FSTL1 Antibody (C-term) AP10534b
    All lanes : Anti-FSTL1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human placenta lysate Lane 3: human heart lysate Lane 4: mouse heart lysate Lane 5: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - FSTL1 Antibody (C-term) AP10534b
    FSTL1 Antibody (C-term) (Cat. #AP10534b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FSTL1 antibody detected the FSTL1 protein (arrow).
  • IHC-P - FSTL1 Antibody (C-term) AP10534b
    FSTL1 antibody (C-term) (Cat. #AP10534b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FSTL1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - FSTL1 Antibody (C-term) AP10534b
    FSTL1 Antibody (C-term) (Cat. #AP10534b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q12841
Other Accession Q9GKY0, NP_009016.1
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 280-308 aa
Additional Information
Other Names Follistatin-related protein 1, Follistatin-like protein 1, FSTL1, FRP
Target/Specificity This FSTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human FSTL1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFSTL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FSTL1
Synonyms FRP
Function May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity).
Cellular Location Secreted.
Tissue Location Overexpressed in synovial tissues from rheumatoid arthritis. EMBL; U06863; AAA66062.1; -; mRNA EMBL; D89937; BAA28707.1; -; mRNA EMBL; AB119283; BAD12167.1; -; Genomic_DNA EMBL; AK289388; BAF82077.1; -; mRNA EMBL; AK291138; BAF83827.1; -; mRNA EMBL; AK300356; BAG62097.1; -; mRNA EMBL; AK312261; BAG35192.1; -; mRNA EMBL; AC063952; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC078982; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471052; EAW79526.1; -; Genomic_DNA EMBL; CH471052; EAW79527.1; -; Genomic_DNA EMBL; BC000055; AAH00055.1; -; mRNA CCDS; CCDS2998.1; -. [Q12841-1] PIR; S51362; S51362 RefSeq; NP_009016.1; NM_007085.4. [Q12841-1] SMR; Q12841; - BioGRID; 116338; 42 IntAct; Q12841; 42 MINT; Q12841; - STRING; 9606.ENSP00000295633; - MEROPS; I01.967; - GlyConnect; 1252; 9 N-Linked glycans (1 site) GlyGen; Q12841; 4 sites, 2 O-linked glycans (1 site) iPTMnet; Q12841; - PhosphoSitePlus; Q12841; - BioMuta; FSTL1; - DMDM; 2498390; - EPD; Q12841; - jPOST; Q12841; - MassIVE; Q12841; - MaxQB; Q12841; - PaxDb; Q12841; - PeptideAtlas; Q12841; - PRIDE; Q12841; - ProteomicsDB; 5127; - ProteomicsDB; 58980; -. [Q12841-1] Antibodypedia; 32794; 373 antibodies Ensembl; ENST00000295633; ENSP00000295633; ENSG00000163430. [Q12841-1] Ensembl; ENST00000424703; ENSP00000394355; ENSG00000163430. [Q12841-2] GeneID; 11167; - KEGG; hsa:11167; - UCSC; uc003eds.4; human. [Q12841-1] CTD; 11167; - DisGeNET; 11167; - EuPathDB; HostDB:ENSG00000163430.9; - GeneCards; FSTL1; - HGNC; HGNC:3972; FSTL1 HPA; ENSG00000163430; Low tissue specificity MIM; 605547; gene neXtProt; NX_Q12841; - OpenTargets; ENSG00000163430; - PharmGKB; PA28389; - eggNOG; ENOG502QQAG; Eukaryota GeneTree; ENSGT00940000157784; - HOGENOM; CLU_038229_0_0_1; - InParanoid; Q12841; - KO; K23912; - OMA; KGSQTHT; - OrthoDB; 1079836at2759; - PhylomeDB; Q12841; - TreeFam; TF106409; - PathwayCommons; Q12841; - Reactome; R-HSA-201451; Signaling by BMP Reactome; R-HSA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) Reactome; R-HSA-8957275; Post-translational protein phosphorylation BioGRID-ORCS; 11167; 1 hit in 871 CRISPR screens ChiTaRS; FSTL1; human GeneWiki; FSTL1; - GenomeRNAi; 11167; - Pharos; Q12841; Tbio PRO; PR:Q12841; - Proteomes; UP000005640; Chromosome 3 RNAct; Q12841; protein Bgee; ENSG00000163430; Expressed in connective tissue and 235 other tissues ExpressionAtlas; Q12841; baseline and differential Genevisible; Q12841; HS GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0005576; C:extracellular region; TAS:Reactome GO; GO:0005615; C:extracellular space; HDA:UniProtKB GO; GO:0005509; F:calcium ion binding; IEA:InterPro GO; GO:0008201; F:heparin binding; TAS:ProtInc GO; GO:0030509; P:BMP signaling pathway; TAS:Reactome GO; GO:0030154; P:cell differentiation; IBA:GO_Central GO; GO:0044267; P:cellular protein metabolic process; TAS:Reactome GO; GO:0007275; P:multicellular organism development; IBA:GO_Central GO; GO:0043687; P:post-translational protein modification; TAS:Reactome GO; GO:0030510; P:regulation of BMP signaling pathway; IBA:GO_Central GO; GO:0042594; P:response to starvation; IEA:Ensembl Gene3D; 3.90.290.10; -; 1 InterPro; IPR011992; EF-hand-dom_pair InterPro; IPR002048; EF_hand_dom InterPro; IPR003645; Fol_N InterPro; IPR015369; Follistatin/Osteonectin_EGF InterPro; IPR002350; Kazal_dom InterPro; IPR036058; Kazal_dom_sf InterPro; IPR036773; TB_dom_sf Pfam; PF09289; FOLN; 1 Pfam; PF07648; Kazal_2; 1 SMART; SM00274; FOLN; 1 SMART; SM00280; KAZAL; 1 SUPFAM; SSF100895; SSF100895; 1 SUPFAM; SSF47473; SSF47473; 1 PROSITE; PS50222; EF_HAND_2; 2 PROSITE; PS51465; KAZAL_2; 1 1: Evidence at protein level; Alternative splicing; Direct protein sequencing; Disulfide bond; Glycoprotein; Heparin-binding; Phosphoprotein; Reference proteome; Repeat; Secreted; Signal SIGNAL 1..20 /evidence="ECO:0000269|PubMed:15340161" CHAIN 21..308 /note="Follistatin-related protein 1" /id="PRO_0000010111" DOMAIN 30..53 /note="Follistatin-like" DOMAIN 48..100 /note="Kazal-like" /evidence="ECO:0000255|PROSITE-ProRule:PRU00798" DOMAIN 144..178 /note="EF-hand 1" /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" DOMAIN 193..228 /note="EF-hand 2" /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" DOMAIN 233..287 /note="VWFC" MOD_RES 165 /note="Phosphoserine; by FAM20C" /evidence="ECO:0000269|PubMed:26091039" CARBOHYD 144 /note="N-linked (GlcNAc...) asparagine" /evidence="ECO:0000255" CARBOHYD 175 /note="N-linked (GlcNAc...) asparagine" /evidence="ECO:0000269|PubMed:16335952" CARBOHYD 180 /note="N-linked (GlcNAc...) asparagine" /evidence="ECO:0000269|PubMed:16335952" DISULFID 54..84 /evidence="ECO:0000255|PROSITE-ProRule:PRU00798" DISULFID 58..77 /evidence="ECO:0000255|PROSITE-ProRule:PRU00798" DISULFID 66..98 /evidence="ECO:0000255|PROSITE-ProRule:PRU00798" VAR_SEQ 22..56 /note="Missing (in isoform 2)" /evidence="ECO:0000303|PubMed:14702039" /id="VSP_055075" CONFLICT 221 /note="C -> S (in Ref. 4; BAF83827)" /evidence="ECO:0000305" CONFLICT 226 /note="F -> S (in Ref. 4; BAF83827)" /evidence="ECO:0000305" SEQUENCE 308 AA; 34986 MW; BD4C651FAFF24800 CRC64; MWKRWLALAL ALVAVAWVRA EEELRSKSKI CANVFCGAGR ECAVTEKGEP TCLCIEQCKP HKRPVCGSNG KTYLNHCELH RDACLTGSKI QVDYDGHCKE KKSVSPSASP VVCYQSNRDE LRRRIIQWLE AEIIPDGWFS KGSNYSEILD KYFKNFDNGD SRLDSSEFLK FVEQNETAIN ITTYPDQENN KLLRGLCVDA LIELSDENAD WKLSFQEFLK CLNPSFNPPE KKCALEDETY ADGAETEVDC NRCVCACGNW VCTAMTCDGK NQKGAQTQTE EEMTRYVQEL QKHQETAEKT KRVSTKEI
Research Areas
Citations ( 0 )

References

Kai, N., et al. Brain Res. 1073-1074, 60-70 (2006) :
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

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Cat# AP10534b
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